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He sometimes mounted an embryo in copyright balsam and shut it with glass addresses in a very chamber slide to ensure he could look at it from each side (dehydration and clearing were being now founded strategies [forty three, p. twelve]).After the paraffin has drained, open up the retort and take away the basket. Take note the basket should stil
This really is especially legitimate for immunohistochemistry in which ten% NBF has long been shown to be a inadequate fixative (Fig. two). Some problems with the usage of 10% NBF as being a fixative happen to be corrected via the introduction of other experimental techniques including antigen retrieval (Shi et al. 2001) and the usage of modest amp
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